How to use centrifuge

Centrifuge is an instrument often used in many laboratories. It separates substances with different densities and sizes by means of centrifugal force generated by the high-speed rotation of the rotating shaft. It can achieve the purpose of preliminary separation and purification, and is an essential instrument in the laboratory. It can be divided into floor-standing high-speed freezing centrifuge, centrifuge table low speed and so on. (See the classification of centrifuge and centrifuge rotor for details).

? If the centrifuge is used improperly, it will cause accidents and lead to tragedy. How to use it correctly, prolong its service life and minimize accidents? 1: centrifuge placement requirements? Try to choose a dry place and avoid direct sunlight. B. Don't pile up sundries around, and be at least 10cm away from airtight and poor heat dissipation articles such as walls and baffles. C try to put it in a room alone, and don't put organic reagents or flammable materials around it. ? 2. The danger of centrifuge? After the centrifuge starts working, it is a high-speed rotating body. Once an accident happens, it is quite dangerous. The high-speed or ultra-high-speed rotating head flies out and can penetrate almost everything. 3. What are the precautions for using centrifuges? A. According to the centrifuge tubes needed and used in the experiment, special centrifuge tubes should be used for liquids containing organic reagents; B. Select a suitable rotating head, and the rotating speed is absolutely forbidden to exceed the maximum rotating speed indicated by the rotating head; C it is very important to use the centrifugal tube and rotor correctly, especially the symmetrical placement and weight balance of the centrifugal tube; D. Pay attention to the solution with the maximum weight that the centrifugal tube and rotor can bear, and the liquid can fill up to 3/4 of the total volume of the centrifugal tube at most. ? Many accidents are caused by improper use of rotating head and centrifugal tube. ? 4. Maintenance of centrifuge? After each use, the cover of the centrifuge should be opened to allow the heat or water vapor to evaporate naturally. If you use low-temperature centrifugation before, it may freeze. After the ice melts, use dry cotton in time? Scrub the gauze clean, and cover it after there is no obvious water vapor. If the centrifuge rotors can be replaced, each rotor should be taken out in time after use, scrubbed with clean and dry medical gauze and placed upside down. Don't scratch with sharp tools. Aluminum rotors should be cleaned more often. The centrifuge should not be maintained and overhauled frequently, and the power supply should be cut off when the operator leaves. First-time users should consult people who have used it before or mainly refer to the instruction manual. Don't use blindly. ? 5. Frequently asked questions about centrifuges? Common problems are: the cover can't be opened, the centrifuge tube can't be taken out, and the centrifuge doesn't work after pressing the button. The more serious problem is that the rotating shaft is bent due to uneven stress, the motor is burnt out and the horizontal bucket is thrown out. A more serious accident is injury. ? 6. What should I pay attention to in the specific operation? ? When using, pay attention to it from all aspects. After selecting the rotary head and setting the parameters, observe the centrifuge for a period of time, reach the highest speed, and leave after the operation is stable. ? If you hear abnormal sound or smell peculiar smell during operation, you should brake immediately, press the "Stop" button, and cut off the power supply if necessary. Centrifugal tubes must be placed symmetrically, and the weight of corresponding centrifugal tubes should be as equal as possible. Never open the centrifuge cover when the machine is running! At the same time, it is also necessary for all laboratory personnel to develop good registration habits. Enter your answers, and every professional answer will shape your authoritative image.