Do cells that do pcr and wb need to be sterile?

How many cells are needed for WB and RT-PCR at the cellular level, and how large plates are generally used for culture. ...

all

blood

Whole blood (that is, blood that has not been separated in any way)

western

Western western blot is to transfer protein to the membrane and then detect it with antibody. For known expressed proteins, corresponding antibodies can be used as primary antibodies, and for the expression products of new genes, antibodies of fusion parts can be used for detection.

Reverse transcription polymerase chain reaction:

reverse

transcriptase

polymerase

chain

Reverse transcription polymerase chain reaction

The initial template is the PCR reaction of RNA, which requires the initial reverse transcriptase to generate DNA template. Some thermostable polymerases have appropriate reverse transcriptase activity. It is more common to carry out reverse transcription first, then inactivate or purify reverse transcriptase, and then carry out conventional PCR reaction.

IHC:

internal

hair

cell

Inner hair cells (a row of hair cells regularly distributed on the basement membrane near the center of the cochlea)

immunohistochemistry

Immunohistochemistry, using the principle of specific binding between antigen and antibody, makes labeled antibody become chromogenic agent through chemical reaction.

(fluorescein, enzyme, metal ion, isotope)

The intracellular antigens (peptides and protein) in tissues were determined by color development, and their localization, qualitative and quantitative studies were carried out.