Do the abbreviations of genes have to be written in italics?

This is stipulated by the International Naming Committee. Gene names must be expressed in lowercase italics, and the first letter of protein should be capitalized, not italicized. Nowadays, more formal magazines require writing according to regulations.

If known, primers can be designed directly according to the sequences at both ends, and the gene can be amplified directly by PCR. ?

If it is an unknown gene with known function, design primers according to similar genes in similar species, and then carry out PCR. If the gene is unknown or screened, it can be amplified by random primers or assisted by other methods.

Extended data:

RAD-seq has many methods, but the database building process is roughly as follows, and there are differences in some steps between different methods.

Initial genomic DNA quantity: Can FNA be allowed to degrade?

Restriction endonuclease hydrolysis: limiting the types and quantities of enzymes.

Restriction site binding linker: linker type

The selection of the size of the enzymolysis fragment: direct selection and indirect selection.

Add barcode mixing pool: depending on V-joint.

Selection of sequencing types: single-ended and double-ended.

The difference between the two is that,

1) is digested, then randomly broken, and finally only fragments with restriction sites are selected for sequencing;

2) It is the same as enzyme digestion, but the fragments with appropriate size are directly selected for sequencing.

So compared to

The average number of sequencing sites will be less, resulting in the lower utilization rate of the latter than the former in the same batch of samples. Non-reference genomes recommend the former rather than the latter.

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